GO analysis with DEGs

Prepare a list of genes

After users have identified a list of DEGs, users can perform GO pathway enrichment analysis with these DEGs by clicking "Go to pathway analysis".

On the pathway analysis page, users can select all DEGs (the blue box) or select a subset of DEGs based on fold changes or p-values. Users also need to select a gene set for analysis (Molecular function, Biological process or Cellular component) After a gene list and gene sets are specified, users can start GO enrichment analysis by clicking on "Start".

GO enrichment results

TACCO provides the gene ratio distribution plot for enriched GO terms. Users can download, zoom in, zoom out or reset the plot.

TACCO also provides the connected network plot for enriched GO terms. Pathways sharing the same gene(s) are connected in the network plot. Users can also download, zoom in, zoom out or reset the plot.

A directed acyclic graph presents the relationship between enriched GO terms is also generated.


Detailed information for GO enrichment analysis is listed in the table. Users can download the table.

Select another list of genes

Users can also go back to select DEGs all over again by clicking on "Choose another dataset".